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Isolation of Mouse Lung Dendritic Cells
Published on: November 22, 2011
Isolation of mouse thymic dendritic cells
Methods in Molecular Medicine
|March 5, 2011
Abstract:
The method described in this chapter for the isolation of mouse thymic dendritic cells (DC) is an optimization of our previously published methods (1,2) and involves the following major steps: 1. Enzymatic digestion of thymic fragments with collagenase and DNase. 2. Separation of a very-low-density cell fraction (VLDF). 3. Magnetic depletion of T-lineage cells, B cells, macrophages, and granulocytes. 4. Positive selection of DCs by magnetic cell sorting (MACS).
