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Published on: October 18, 2012
Antigen engineering in DNA immunization
1Department of Medicine, University of Massachusetts Medical Center, Worcester, MA.
Abstract:
J. A. Wolff and colleagues demonstrated in 1990 (1) that naked, non-infectious DNA plasmids can be transferred into muscle cells of living mice to express the encoded protein in vivo. Further studies have shown that proteins expressed in vivo via such a "DNA inoculation" process can serve as immunogens to effectively stimulate the body's immune system to produce potentially strong, long-lasting, antigen-specific, humoral and cell-mediated immune responses (2-8).
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