Construction of Recombinant Adeno-Associated Virus (AAV)

Efficient and stable transfer of foreign DNA into cells both in vitro and in vivo has become a powerful tool in the study of the pathogeneses of various diseases, such as cancer and infectious diseases. The study of hepatitis C virus (HCV) pathogenesis has been hampered by the lack of an easily available animal model or in vitro replication system. A new approach to the establishment of an HCV model are cell-culture systems stably expressing viral genes.