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Updated: Jun 3, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Detection Methods for Glycopeptide-Resistant Staphylococcus aureus II : Cell Wall Analysis.

H Hanaki¹, K Hiramatsu

¹Department of Bacteriology, Juntendo University, Bunkyo-ku, Tokyo, Japan.

Methods in Molecular Medicine

|March 5, 2011

Summary

This summary is machine-generated.

Vancomycin resistance in Staphylococcus aureus involves altered cell-wall synthesis. Measuring cell-wall precursor uptake and murein monomer precursor (MMP) levels helps understand this resistance mechanism.

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Last Updated: Jun 3, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Area of Science:

Microbiology
Biochemistry
Molecular Biology

Background:

Vancomycin resistance in Staphylococcus aureus (e.g., VRSA Mu50, hetero-VRSA Mu3) is linked to alterations in cell-wall synthesis.
Understanding these changes is crucial for elucidating resistance mechanisms.

Purpose of the Study:

To investigate the role of cell-wall synthesis and composition in vancomycin resistance.
To analyze the significance of murein monomer precursor (MMP) levels in altered cell-wall synthesis.

Main Methods:

Evaluating cell-wall synthesis rates by measuring (14)C-N-acetylglucosamine uptake.
Detecting and quantifying the murein monomer precursor (UDP-N-acetylmuramyl-pentapeptide) (MMP).

Main Results:

Increased cytoplasmic pool size of MMP observed in vancomycin-resistant strains (Mu50, Mu3) compared to susceptible strains.
Cell-wall synthesis and composition are key factors in glycopeptide resistance.

Conclusions:

MMP levels serve as a valuable indicator for investigating alterations in cell-wall synthesis associated with vancomycin resistance in S. Aureus.
Further analysis of cell-wall synthesis and composition is vital for understanding and combating antibiotic resistance.