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Amplification of lectin-gold histochemistry.
J F Madrld1, F Hernández, J Ballesta
1Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Leioa, Vizcaya, Spain.
Methods in Molecular Medicine
|March 5, 2011
Summary
Early microscopy techniques utilized colloidal gold probes. A photochemical method, predating its common use, visualized tissue-gold interactions in animals by depositing silver around gold particles.
Area of Science:
- Biomedical imaging
- Materials science
Background:
- Colloidal gold emerged as a microscopy probe in the late 1970s.
- Roberts previously employed gold salts in vivo, visualizing tissue-gold interactions.
- This visualization utilized a photochemical method based on Liesegang and Rieder's photographic process.
Purpose of the Study:
- To explore the historical development of gold-based microscopy probes.
- To detail Roberts's early in vivo application of gold salts for tissue analysis.
Main Methods:
- Photochemical deposition of metallic silver around gold particles.
- Microscopic detection of tissue-gold interactions in living animals.
Main Results:
- Successful visualization of tissue-gold interactions using a photochemical silver enhancement method.
- Demonstration of in vivo gold salt detection predating widespread colloidal gold probe use.
Conclusions:
- Roberts's photochemical method provided early microscopic insights into tissue-gold interactions.
- This technique laid groundwork for later developments in gold-based imaging probes.
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