Applications and recent developments of multi-analyte simultaneous analysis by enzyme-linked immunosorbent assays

Area of Science:

• Analytical Chemistry
• Biochemistry
• Immunotechnology

Background:

• Enzyme-linked immunosorbent assays (ELISAs) are widely used for detecting organic compounds.
• Simultaneous analysis of multiple analytes offers efficiency and comprehensive profiling.
• Existing ELISA methods often focus on single analytes, limiting throughput.

Purpose of the Study:

• To review the development and application of multi-analyte simultaneous analysis by ELISAs.
• To provide a comprehensive reference for researchers in the field.
• To highlight key considerations for designing effective multi-analyte ELISAs.

Main Methods:

• Detailed description of multi-analyte ELISAs utilizing generic antibodies.
• Explanation of strategies involving specific antibodies, including multi-antibody co-coating and multi-tracer co-marking.
• Focus on the design and synthesis of generic haptens and their conjugation to carrier proteins.

Main Results:

• Successful implementation of multi-analyte ELISAs for various organic compounds.
• Demonstration of different approaches for achieving simultaneous detection.
• Identification of critical factors influencing assay performance, such as antibody characteristics and ELISA format.

Conclusions:

• Multi-analyte simultaneous analysis by ELISAs is a rapidly advancing field.
• The review provides valuable insights into the design and application of these assays.
• This work serves as a reference for future investigations in multi-analyte ELISA development.