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Generating Genetically Modified Plasmodium berghei Sporozoites
Published on: May 5, 2023
Characterization of Plasmodium berghei glutathione synthetase
1Department of Biosciences, Himachal Pradesh University, Shimla, India. sanjaysharma_bio@yahoo.com
Abstract:
Plasmodium berghei contained 0.454±0.031 U/mg of glutathione synthetyase (GS). GS was purified using solid ammonium sulfate and Sephadex G-200 from P. berghei infected mouse erythrocytes. SDS-PAGE showed purified GS as a single band protein of 70 kDa and its Km for γ-glutamylcysteine, glycine and ATP being 0.33 mM, 8.3 mM and 0.43 mM respectively with noncompetitive inhibition by GSH. The malaria parasite enzyme was optimally active at 37°C and pH 8.0-8.5. Heavy metals significantly inhibited parasite GS activity.
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