Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with...

Lambda gt11 cDNA libraries are routinely used for gene isolation with antibody and oligomer probes (1-3). Briefly, a recombinant DNA library from different types of cells is constructed in a λgt 11 expression vector. The foreign peptides (antigens) are expressed as a β-galactosidase fusion protein in E. coli. Because the lacZ gene has a strong promoter, the fusion proteins comprise from 0.1-4% of the total E. coli proteins (1-3). The expressed fusion antigens are transferred onto nitrocellulose filters and then probed with antibodies to identify the targets containing the specific, cognate epitope.