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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jun 3, 2026

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
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Serologic analysis by enzyme-linked immunosorbent assay (ELISA).

M Krider1, L D Loomis-Price

  • 1Henry M. Jackson Foundation, Rockville, MD.

Methods in Molecular Medicine
|March 8, 2011
PubMed
Summary

Measuring vaccine immunogenicity is crucial. The enzyme-linked immunosorbent assay (ELISA) is a standard method for quantifying antibody responses against protein antigens, vital for vaccine development and monitoring.

Area of Science:

  • Immunology
  • Vaccinology
  • Biotechnology

Background:

  • Assessing vaccine candidate efficacy requires measuring immunogenicity.
  • Immunogenicity assessment involves evaluating humoral and/or cellular immune responses.
  • Quantifying antibody levels against specific antigens is essential for humoral response evaluation.

Purpose of the Study:

  • To highlight the importance of measuring immunogenicity for vaccine candidates.
  • To introduce the enzyme-linked immunosorbent assay (ELISA) as a key method for antibody detection.

Main Methods:

  • The study focuses on the enzyme-linked immunosorbent assay (ELISA) technique.
  • ELISA is used to measure antibody levels against protein antigens.
  • This method is applicable to monitoring responses after infection or vaccination.

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Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
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Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
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Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

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Main Results:

  • ELISA provides a quick and accurate method for antibody quantification.
  • The assay is a long-standing tool in vaccine development.
  • ELISA is routinely employed for monitoring immune responses to HIV proteins.

Conclusions:

  • Accurate immunogenicity assessment is fundamental for vaccine development.
  • Enzyme-linked immunosorbent assay (ELISA) is a critical tool for measuring antibody responses to protein antigens.
  • ELISA facilitates the monitoring of vaccine efficacy and immune status.