[Determination of three adrenergic drugs using capillary electrophoresis with amperometric detection]

A method for the determination of three adrenergic drugs, including phenylephrine hydrochloride (PHE), metaraminol bitartrate (MR) and isoprenaline hydrochloride (IP), was developed using capillary electrophoresis with amperometric detection. The detection potential of working electrode was 0.950 V versus the reference electrode of Ag/AgCl. At the applied voltage of 18 kV, the three analytes were completely separated within 18 min in 50 mmol/L borate buffer (pH 10.00) with the injection time of 10 s. Good linear relationships were obtained for all the three analytes in the range of 2-100 micromol/L. The detection limits for PHE, MR and IP were 0.8, 0.8 and 1.0 micromol/L, respectively. The proposed method was applied to the analysis of some injection drugs, and the results were satisfactory.