Diagnostic potential of Stokes Shift spectroscopy of breast and prostate tissues-- a preliminary pilot study

Stokes Shift (SS) Spectroscopy (SSS) of normal and abnormal breast and prostate tissues were studied. SS spectra is measured by simultaneously scanning both the excitation and emission wavelengths while keeping a fixed wavelength interval of Δλ = 20 nm. Characteristic, highly resolved peaks and significant spectral differences between normal and different pathological tissues of breast and prostate tissues were observed. The SS spectra of normal and different pathological breast and prostate tissues show the distinct peaks around 300, 350, 450, 500 and 600 nm may be attributed to tryptophan, collagen, NADH, flavin and porphyrin, respectively. Results of the current study demonstrate that the SS spectral changes due to tryptophan, collagen, hemoglobin, NADH, FAD and porphyrin have good diagnostic potential; therefore can be targeted as native tumor markers.