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07:43
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Enterohepatic helicobacter in ulcerative colitis: potential pathogenic entities?
John M Thomson1, Richard Hansen, Susan H Berry
1Gastrointestinal Research Group, Division of Applied Medicine, University of Aberdeen, Foresterhill, Aberdeen, United Kingdom.
Plos One
|March 9, 2011
Summary
Novel Helicobacter species are more prevalent in ulcerative colitis (UC) patients. This study developed molecular methods to detect these bacteria, suggesting Helicobacter may play a role in UC pathogenesis.
Area of Science:
- Microbiology
- Gastroenterology
- Molecular Biology
Background:
- Ulcerative colitis (UC) pathogenesis is linked to gut dysbiosis.
- The role of novel Helicobacter species in human UC remains debated and under-investigated.
Purpose of the Study:
- To develop and validate a molecular approach for detecting Helicobacter organisms in adults with and without UC.
Main Methods:
- Developed a dual molecular detection strategy using genus-specific Helicobacter probes and validated H. pylori probes.
- Utilized PCR and Fluorescence In Situ Hybridization (FISH) on colonic samples from UC patients and non-IBD controls.
- Sequenced positive PCR samples and statistically analyzed the association between Helicobacter and study groups.
Main Results:
- Helicobacter genus PCR positivity was significantly higher in UC patients (32/77) compared to controls (11/59).
- Enterohepatic Helicobacter species prevalence was significantly higher in the UC group (30/77) versus controls (2/59).
- PCR and FISH showed concordance in 67.9% of subjects, with FISH yielding higher positivity rates.
Conclusions:
- Helicobacter organisms should be considered potential contributors to UC pathogenesis.
- Further isolation of these organisms from colonic tissue is necessary to confirm pathogenicity.
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