Production of haloduracin by Bacillus halodurans using solid-state fermentation
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High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies
Published on: September 29, 2023
Abolghasem Danesh1, Gashaw Mamo, Bo Mattiasson
1Department of Biotechnology, Lund University, Lund, Sweden.
Abstract:
Bacillus halodurans was cultivated on wheat bran as a solid-state substrate and produced haloduracin, a bacteriocin, at about 245 AU per wheat bran. Supplementation of the bran with Lauria-Bertani broth decreased haloduracin production. However, production was stimulated by addition of Mg(2)SO(4) and K(2)HPO(4). The highest production was achieved at a wheat bran/moisture ratio of 1:1.8 and in the presence of 10% (w/w) Na(2)CO(3). Under optimum conditions, the organism produced about 3,000 AU per gram dry bran.
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