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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Nanoarray-based biomolecular detection using individual Au nanoparticles with minimized localized surface plasmon
Longhua Guo1, Abdul Rahim Ferhan, Kijoon Lee
1School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore.
Analytical Chemistry
|March 11, 2011
Summary
We developed a method to create 2D plasmonic nanoarrays using individual metallic nanoparticles. This platform enables consistent detection of refractive index changes and biomolecular binding events on nanoparticles.
Area of Science:
- Nanotechnology
- Plasmonics
- Optical Sensing
Background:
- Individual metallic nanoparticles exhibit localized surface plasmon resonance (LSPR) for sensing applications.
- Scaling LSPR sensing to nanoarrays requires robust signal normalization and consistent detection.
Purpose of the Study:
- To develop a method for expanding the use of individual metallic nanoparticles into 2D plasmonic nanoarrays.
- To establish an optical detection platform for tracking LSPR signals from individual nanoparticles within nanoarrays.
- To demonstrate a normalization method for LSPR signals from geometrically diverse nanoparticles.
Main Methods:
- Constructed an optical detection platform to scan and reconstruct pseudoimages of nanoparticles from scattering spectra.
- Developed a normalization technique to standardize LSPR signals from nanoparticles of varying geometry.
- Validated the system by measuring aptamer-thrombin binding events.
Main Results:
- Achieved consistent plasmonic responses from normalized LSPR signals across different nanoparticles.
- Demonstrated well-fitted dose-response curves for refractive index changes and receptor-analyte binding.
- Successfully implemented a proof-of-concept plasmonic nanoarray for aptamer-thrombin detection.
Conclusions:
- The developed method enables the creation and detection of 2D plasmonic nanoarrays.
- Normalization of LSPR signals is crucial for consistent and reliable sensing with diverse nanoparticles.
- The platform shows promise for sensitive detection of biomolecular interactions.
