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Related Experiment Video

Updated: Jun 3, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

A nanoparticle-based sensor for visual detection of multiple mutations.

Dimitrios S Elenis1, Penelope C Ioannou, Theodore K Christopoulos

  • 1Department of Chemistry, University of Athens, Athens 15771, Greece.

Nanotechnology
|March 11, 2011
PubMed
Summary

Disposable DNA biosensors on lateral flow strips offer simple, instrument-free visual detection for genotyping. This study enhances their multiplexing ability for efficient multiallele detection in field or lab settings.

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Area of Science:

  • Biotechnology
  • Genetics
  • Biosensor Technology

Background:

  • Lateral flow strip biosensors offer simple, low-cost, and portable DNA genotyping.
  • Current limitations include challenges in multiplexing capabilities.
  • Dry-reagent formats minimize complex laboratory procedures.

Purpose of the Study:

  • To enhance the multiplexing capabilities of lateral flow strip DNA biosensors.
  • To enable rapid, instrument-free visual detection of multiple DNA alleles.
  • To demonstrate a novel approach for multiallele genotyping using enhanced lateral flow strips.

Main Methods:

  • Utilizing polymerase chain reaction (PCR) followed by a single primer extension reaction for all target alleles.
  • Incorporating biotin during primer extension for perfect complementarity to target alleles.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Related Experiment Videos

Last Updated: Jun 3, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

  • Employing polystyrene microspheres functionalized with capture probes on multiple test spots for multiallele detection.

    • Main Results:

    • Demonstrated successful multiallele detection through specific hybridization of primer extension products to capture probes.
    • Achieved enhanced detectability by immobilizing microspheres on the membrane, preventing diffusion and improving hybrid exposure.
    • Validated the specificity and performance of the developed biosensor for multiallele genotyping.

    Conclusions:

    • The enhanced lateral flow strip biosensor significantly improves multiplexing capabilities for DNA genotyping.
    • This approach offers a simple, rapid, and instrument-free method for visual detection of multiple DNA alleles.
    • The developed biosensor is suitable for field testing and small laboratory applications requiring efficient multiallele genotyping.