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In vivo Dual Substrate Bioluminescent Imaging
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Published on: October 11, 2011

Streptavidin-aequorin fusion protein for bioluminescent immunoassay

Satoshi Inouye1, Jun-ichi Sato, Satoko Sasaki

  • 1Yokohama Research Center, Chisso Corporation, Yokohama, Japan. sinouye@chisso.co.jp

Bioscience, Biotechnology, and Biochemistry
|March 11, 2011
PubMed

The fusion protein of streptavidin to aequorin (STA-AQ) was highly purified from inclusion bodies in Escherichia coli cells and applied to a bioluminescent sandwich immunoassay. α-Fetoprotein (AFP), which is a serological marker of liver cancer, was used as a model analyte to test STA-AQ in an immunoassay. The measurable range of AFP by the sandwich immunoassay, using the complex of STA-AQ and the biotinylated anti-AFP antibody, was 0.02-200 ng/mL with an average coefficient of variation of 4.9%. The detection sensitivity with the complex of STA-AQ and the biotinylated anti-AFP antibody was similar to that with the complex of biotinylated aequorin, streptavidin and the biotinylated anti-AFP antibody. STA-AQ would be a useful reporter protein for immunoassays.

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