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Related Experiment Video

Updated: Jun 3, 2026

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
05:28

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Published on: August 19, 2021

The light responsive transcriptome of the zebrafish: function and regulation.

Benjamin D Weger1, Meltem Sahinbas, Georg W Otto

  • 1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.

Plos One
|March 11, 2011
PubMed
Summary

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Zebrafish light exposure induces 117 genes, impacting circadian rhythms, stress, and DNA repair. Specific DNA sequences mediate this light-induced gene expression, suggesting a general regulatory pathway.

Area of Science:

  • * Molecular Biology
  • * Chronobiology
  • * Genetics

Background:

  • * Organisms use circadian clocks to anticipate daily cycles, entrained by ambient light.
  • * Zebrafish exhibit direct light responsiveness in organs and cell lines, enabling clock entrainment.
  • * Light-induced gene transcription is a key mechanism for circadian clock regulation.

Purpose of the Study:

  • * To characterize light-induced gene transcription in zebrafish across multiple organizational levels.
  • * To identify genes regulated by light pulses and understand their functions.
  • * To investigate the molecular mechanisms underlying light-mediated gene regulation in zebrafish.

Main Methods:

  • * Exposure of zebrafish larvae, heart organ cultures, and cell cultures to 1- or 3-hour light pulses.

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Related Experiment Videos

Last Updated: Jun 3, 2026

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
05:28

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Published on: August 19, 2021

A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities
09:39

A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities

Published on: October 3, 2008

Optogenetic Signaling Activation in Zebrafish Embryos
07:18

Optogenetic Signaling Activation in Zebrafish Embryos

Published on: October 27, 2023

  • * Comparison of gene expression changes between light-exposed and dark-control groups.
  • * Promoter analysis, heterologous reporter constructs, and Morpholino-mediated knock-down studies.

    • Main Results:

    • * Identified 117 light-regulated genes, with most induced and some repressed by light.
    • * Cluster analysis revealed five gene classes regulated at different organizational levels.
    • * Enriched gene ontology categories include circadian rhythms, stress response, and DNA repair.
    • * Promoter analysis identified E- and D-box enhancers involved in light regulation.
    • * D-box elements showed light regulation in both cells and larvae.
    • * Knock-down studies indicated differential involvement of Tef homologues in light induction.

    Conclusions:

    • * Light exposure triggers a broad transcriptional response in zebrafish, affecting diverse cellular functions.
    • * The identified regulatory motifs and pathways suggest a general mechanism for light-induced gene expression.
    • * Findings provide insights into how light influences circadian rhythms, stress responses, and DNA repair.
    • * The study highlights the period2 gene's regulatory pathway as a potential general model for light-mediated gene induction.