Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms00:53

Transgenic Organisms

Overview
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hormonal control of growth and development.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Plant protoplast enucleation by density gradient centrifugation.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Transformation of Rape with Agrobacterium tumeficiens-Based Vectors.

Methods in molecular biology (Clifton, N.J.)·2011
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
08:31

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

Agrobacterium Rhizogenes-Mediated Gene Transfer Using PRI 1855 and a Binary Vector.

H K Fakhrai1

  • 1University of Durham, Durham, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary

Agrobacterium rhizogenes induces hairy root disease in plants by integrating T-DNA. This bacterium offers a promising alternative for plant transformation due to efficient regeneration of transformed cells.

More Related Videos

Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Related Experiment Videos

Last Updated: Jun 3, 2026

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
08:31

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Area of Science:

  • Plant pathology
  • Molecular biology
  • Biotechnology

Background:

  • Agrobacterium rhizogenes causes hairy root disease in dicotyledonous plants.
  • The disease involves T-DNA transfer from the Ri-plasmid into the plant genome, leading to root proliferation.

Purpose of the Study:

  • To highlight the growing interest in Agrobacterium rhizogenes for plant transformation.
  • To suggest Agrobacterium rhizogenes as a potential alternative to Agrobacterium tumefaciens Ti-vector systems.

Main Methods:

  • Review of recent research on Agrobacterium rhizogenes-mediated plant transformation.
  • Analysis of the regeneration capabilities of Ri-T-DNA transformed plant cells.

Main Results:

  • Agrobacterium rhizogenes facilitates the transfer and integration of T-DNA into the host plant genome.
  • Transformed plant cells from certain species can be regenerated into whole plants with relative ease.

Conclusions:

  • The efficient regeneration of Ri-T-DNA transformed cells makes Agrobacterium rhizogenes a valuable tool.
  • An Agrobacterium rhizogenes Ri-vector system may serve as a useful alternative to traditional Ti-vector systems for plant biotechnology.