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Related Concept Videos

Fed-Batch Culture01:23

Fed-Batch Culture

Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
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Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

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An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
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Batch suspension culture.

P A Bond1

  • 1Bristol Polytechnic, Bristol Polytechnic, Frenchay, Bristol, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Batch culture is a method where cells grow in a fixed volume of medium, with no additions except for pH control. The final biomass is harvested after the growth cycle is complete.

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Area of Science:

  • Biotechnology
  • Microbiology
  • Bioprocess Engineering

Background:

  • Batch culture is a fundamental bioprocessing technique.
  • It involves inoculating a set volume of medium with cells for growth.
  • This method is widely used in various biotechnological applications.

Purpose of the Study:

  • To describe the principles and methodology of batch culture.
  • To highlight key operational parameters and outcomes.
  • To provide a foundational understanding of this microbial cultivation technique.

Main Methods:

  • Inoculation of a defined volume or mass of cells into a fixed volume of medium.
  • Allowing microbial growth to proceed without further nutrient addition.
  • Monitoring and controlling parameters such as pH using acid/alkali additions.
  • Harvesting the resulting biomass at a desired stage of the growth cycle.

Main Results:

  • Successful cultivation of microbial biomass within a closed system.
  • Biomass yield is dependent on initial conditions and growth duration.
  • The process is scalable based on operational requirements.

Conclusions:

  • Batch culture is a straightforward and versatile method for microbial cultivation.
  • It allows for controlled growth and harvesting of biomass.
  • The technique's simplicity and scalability make it suitable for diverse applications.