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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
Transgenic Organisms00:53

Transgenic Organisms

Overview
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...

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Related Experiment Video

Updated: Jun 3, 2026

Embryo Microinjection for Transgenesis in Drosophila
05:32

Embryo Microinjection for Transgenesis in Drosophila

Published on: June 7, 2024

Introduction to transgenesis.

D Murphy1, D A Carter

  • 1Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Scientists can now insert cloned DNA sequences into animals, creating transgenic organisms. These animals stably pass the new DNA through generations, allowing study of gene function and regulation within the whole organism.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Techniques for introducing cloned DNA into animal germ lines have advanced significantly over the last decade.
  • These introduced DNA sequences, termed transgenes, are stably inherited across generations.

Purpose of the Study:

  • To enable the analysis of specific cloned genes within a whole organism.
  • To investigate the role and regulation of gene expression in vivo.

Main Methods:

  • Introduction of defined, cloned DNA sequences into animal germ lines.
  • Generation of genetically modified animals carrying these sequences.

Main Results:

  • Transgenes are stably integrated and passed to subsequent generations.
  • Transgenes are expressed and exhibit correct tissue-specific, developmental, and physiological regulation.

Conclusions:

  • The development of transgenic organisms provides a powerful tool for studying gene function and regulation.
  • Transgenic technology allows for in-depth analysis of gene roles within the complex biological system of an entire organism.