Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2026

Assessment of Sexual Behavior of Male Mice
04:38

Assessment of Sexual Behavior of Male Mice

Published on: March 5, 2020

Mating mice.

D Murphy1

  • 1Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Mating is essential for generating transgenic mice, producing eggs for microinjection, and creating surrogate mothers. Superovulation significantly boosts egg yield, while some transgenic lines require intervention for reproductive issues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The functional outcome and recovery of patients admitted to an intensive care unit following drug overdose: a follow-up study.

Anaesthesia and intensive care·2009
Same author

Traveller health: prevalence of diabetes, pre diabetes and the metabolic syndrome.

Irish medical journal·2009
Same author

The prevalence and distribution of Mycobacterium bovis infection in European badgers (Meles meles) as determined by enhanced post mortem examination and bacteriological culture.

Research in veterinary science·2009
Same author

Effects of estrogen therapy on age-related differences in gray matter concentration.

Climacteric : the journal of the International Menopause Society·2009
Same author

Neuroleptics in the treatment of aggressive challenging behaviour for people with intellectual disabilities: a randomised controlled trial (NACHBID).

Health technology assessment (Winchester, England)·2009
Same author

Entire genome sequences of two new HCV subtypes, 6r and 6s, and characterization of unique HVR1 variation patterns within genotype 6.

Journal of viral hepatitis·2009
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Area of Science:

  • Animal models
  • Genetics
  • Reproductive biology

Background:

  • Transgenic mouse production relies on controlled breeding for critical steps.
  • Natural matings provide eggs and surrogate mothers, but yields can be limited.
  • Some transgenic strains exhibit reproductive defects impacting colony expansion.

Purpose of the Study:

  • To outline the necessity of animal matings in transgenic mouse creation and analysis.
  • To detail methods for optimizing egg yield for microinjection.
  • To address challenges in maintaining transgenic lines due to reproductive issues.

Main Methods:

  • Utilizing natural matings between mature mice for egg production.
  • Employing superovulation in immature females to increase egg yield significantly.

More Related Videos

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
06:38

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Published on: October 13, 2018

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods
08:28

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods

Published on: March 10, 2020

Related Experiment Videos

Last Updated: Jun 3, 2026

Assessment of Sexual Behavior of Male Mice
04:38

Assessment of Sexual Behavior of Male Mice

Published on: March 5, 2020

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
06:38

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Published on: October 13, 2018

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods
08:28

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods

Published on: March 10, 2020

  • Preparing pseudopregnant females as recipients for microinjected eggs.
  • Implementing assisted reproductive technologies like superovulation or in vitro fertilization for problematic transgenic lines.

    • Main Results:

    • Natural matings yield approximately 10 F2 eggs per mouse.
    • Superovulation can increase egg yield by three- to fivefold.
    • Reproductive defects are common in some transgenic strains, necessitating intervention.

    Conclusions:

    • Strategic mating is fundamental for successful transgenic mouse generation.
    • Superovulation is a key technique for enhancing the efficiency of egg collection.
    • Assisted reproductive methods are crucial for preserving and expanding transgenic lines with inherent reproductive challenges.