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Vasectomizing a mouse.

D Murphy1

  • 1Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
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Vasectomized male mice are crucial for inducing pseudopregnancy in females, enabling them to serve as recipients for early-stage embryo transfers. This method ensures successful pregnancy without sperm transmission.

Area of Science:

  • Reproductive biology
  • Mammalian embryology
  • Assisted reproductive technologies

Background:

  • Pseudopregnancy in female mice is essential for successful embryo implantation.
  • Vasectomized males are commonly used to induce pseudopregnancy by mating.
  • This process allows for the transfer of fertilized eggs into a receptive uterine environment.

Purpose of the Study:

  • To outline the necessity and methodology of using vasectomized males in reproductive studies.
  • To establish a reliable method for creating pseudopregnant female mice for egg transfer.
  • To highlight the role of induced pseudopregnancy in assisted reproduction.

Main Methods:

  • Utilizing vasectomized male mice for mating with mature female mice.
  • Confirming pseudopregnancy through the presence of copulatory plugs.

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Last Updated: Jun 3, 2026

Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model
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Published on: February 28, 2014

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08:49

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  • Employing these pseudopregnant females as recipients for microinjected fertilized one-cell eggs.

    • Main Results:

    • Vasectomized males successfully induce pseudopregnancy without transmitting sperm.
    • Pseudopregnant females demonstrate hormonal and physiological readiness for embryo implantation.
    • This technique provides a viable model for studying early embryonic development and transfer.

    Conclusions:

    • Vasectomized males are indispensable for inducing pseudopregnancy in mice.
    • This method is critical for creating competent recipients for embryo microinjection and transfer.
    • Alternative methods using genetically sterile males can also be employed.