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Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Updated: Jun 3, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Northern blotting.

¹Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)

|March 11, 2011

Summary

This summary is machine-generated.

Northern blotting analyzes RNA by size and hybridization, enabling transgene expression studies. Modifications ensure transgene RNA is distinguishable from endogenous RNA for accurate analysis.

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Last Updated: Jun 3, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

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Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Area of Science:

Molecular Biology
Genetics

Background:

Northern blotting is a key technique for RNA analysis.
It enables the study of gene expression patterns.

Purpose of the Study:

To detail the Northern blotting technique for analyzing transgene expression.
To highlight methods for distinguishing transgene RNA from endogenous RNA.

Main Methods:

Denaturing RNA and fractionating it by size using agarose gel electrophoresis.
Transferring RNA to a solid matrix for hybridization with a labeled probe.
Modifying transgenes or using specific probes to differentiate RNA molecules.

Main Results:

Northern blotting can reveal transgene expression patterns.
Changes in expression can be tracked during physiological and developmental transitions.
Successful differentiation between transgene and endogenous RNA is crucial.

Conclusions:

Northern blotting is a versatile tool for studying transgene expression.
Careful experimental design is necessary to distinguish transgene from endogenous RNA.
This technique aids in understanding gene function and regulation.