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Selectins

Selectins

Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...

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Related Experiment Video

Updated: Jun 3, 2026

Development of a Negative Selectable Marker for Entamoeba histolytica

Development of a Negative Selectable Marker for Entamoeba histolytica

Published on: December 12, 2010

Selectable markers for eukaryotic cells.

¹Chester Beatty Laboratories, Institute of Cancer Research, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 11, 2011

Summary

This summary is machine-generated.

Efficiently transferring DNA into cells requires selection methods. Selectable markers are crucial for identifying and isolating successfully transfected cells, especially when the DNA doesn't offer a growth advantage.

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Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

DNA sequence transfer into cells (transfection) is rarely 100% efficient, often transducing only 0.1-1% of cells.
When transferred sequences lack a selective growth advantage, selection is essential for identifying modified cells.

Purpose of the Study:

To highlight the necessity of selection methods in cell transfection.
To explain the role and importance of selectable markers in molecular biology.

Main Methods:

Discussion of the calcium phosphate transfection method and its typical efficiency.
Explanation of positive selection strategies using marker genes.

Main Results:

Transfection efficiencies are often low, necessitating robust selection techniques.
Selectable markers enable the identification of cells that have successfully incorporated foreign DNA.

Conclusions:

Selectable markers are critical tools for isolating successfully transfected cells.
Positive selection ensures the survival of desired cells, unlike negative selection methods that eliminate cells.