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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell-surface Signaling01:21

Cell-surface Signaling

Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
What is Cell Signaling?02:03

What is Cell Signaling?

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
What is Cell Signaling?02:03

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Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
Cell Lines01:16

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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
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Published on: February 25, 2017

COS Cell Expression.

J F Hancock1

  • 1Department ofHaematology, Royal Free Hospital School of Medicine, Hampstead, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

COS-1 cells, derived from monkey epithelial cells, support the replication of Simian virus 40 (SV40) related plasmids. This enables transient, high-level expression of proteins encoded by these plasmids.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Virology

Background:

  • COS-1 cells are a derivative of the CV-1 monkey epithelial cell line.
  • These cells were generated through transformation with a defective Simian virus 40 (SV40) mutant.
  • The SV40 mutant contained a deletion in the origin of replication, preventing infectious virus production.

Purpose of the Study:

  • To characterize the COS-1 cell line and its properties.
  • To demonstrate the utility of COS-1 cells for supporting viral genome replication.
  • To establish COS-1 cells as a system for transient protein expression.

Main Methods:

  • Transformation of CV-1 cells with a defective SV40 mutant.
  • Characterization of the resulting COS-1 cell line, including analysis of SV40 genome content.
  • Transfection of COS-1 cells with plasmids containing SV40 origins of replication and eukaryotic promoters.

Main Results:

  • COS-1 cells contain the complete early region of the SV40 genome and express necessary replication proteins.
  • These cells efficiently support the replication of SV40 early region mutants.
  • Plasmids with an SV40 origin replicate to high copy numbers in COS-1 cells, leading to transient high-level protein expression.

Conclusions:

  • COS-1 cells are a valuable tool for studying SV40 replication.
  • The system facilitates transient, high-level expression of proteins encoded by transfected plasmids.
  • This cell line provides a robust platform for molecular biology and gene expression studies.