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Updated: Jun 3, 2026
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Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
Design and construction of poliovirus epitope expression vectors
K L Burke1, J W Almond, D J Evans
1Institut für Mikrobiologie der Universitat Ulm, Abteilung Virologie, Ulm, Federal Republic of Germany.
Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
Abstract:
We have developed the very safe and efficacious live-attenuated Sabin 1 poliovirus vaccine strain as a vehicle for the presentation of defined epitopes from foreign pathogens (1-3). Precise modification of the poliovirus capsid is made possible by the application of recombinant DNA technology to cloned cDNA copies of the viral genome, which are infectious for mammalian cells in culture (4).
