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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
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Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

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Baculovirus expression vectors : choice of expression vector.

V C Emery1

  • 1Department of Virology, Royal Free Hospital School of Medicine, Hampstead, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Baculoviruses are powerful expression vectors for producing eukaryotic proteins. This work details methods for creating recombinant baculoviruses and processing the expressed proteins for various applications.

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Area of Science:

  • Molecular Virology
  • Biotechnology
  • Protein Expression Systems

Background:

  • Baculoviruses are established pest-control agents.
  • Their utility extends to molecular virology as expression vectors.
  • Pioneering work by Max Summers and modifications by David Bishop and Bob Possee advanced the system.

Purpose of the Study:

  • To provide a practical account of baculoviruses as expression vectors.
  • To detail methodologies for gene insertion and recombinant generation.
  • To outline downstream processing of baculovirus-produced proteins.

Main Methods:

  • Construction of single and multiple baculovirus expression vectors.
  • Development of baculovirus transfer vectors for gene insertion.
  • Generation and selection of recombinant baculoviruses.
  • Methods for downstream processing of expressed proteins.

Main Results:

  • Established baculovirus expression systems for eukaryotic protein production.
  • Developed modified vectors for enhanced utility.
  • Provided comprehensive methodologies for vector construction and protein processing.

Conclusions:

  • Baculovirus expression systems offer a robust platform for eukaryotic protein production.
  • The described methodologies facilitate the generation and application of recombinant baculoviruses.
  • This work serves as a practical guide for researchers utilizing baculoviruses as expression vectors.