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PCR

PCR

PCR - Polymerase Chain Reaction

PCR - Polymerase Chain Reaction

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Related Experiment Video

Updated: Jun 3, 2026

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

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Published on: May 22, 2012

Setting up a PCR laboratory.

¹Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.

Methods in Molecular Medicine

|March 11, 2011

Summary

This summary is machine-generated.

High sensitivity in Polymerase Chain Reaction (PCR) can lead to false positives from contamination. Strict contamination avoidance is crucial for reliable diagnostic results in PCR laboratories.

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Area of Science:

Molecular Biology
Biotechnology

Background:

Polymerase Chain Reaction (PCR) offers remarkable sensitivity.
This high sensitivity is also a primary source of error, leading to false-positive results.
Exogenous contamination is a significant challenge in PCR applications.

Purpose of the Study:

To highlight the critical importance of contamination avoidance in Polymerase Chain Reaction (PCR).
To emphasize the need for stringent protocols in PCR laboratory setup for diagnostic purposes.

Main Methods:

The study focuses on the principles of PCR and contamination sources.
It reviews standard laboratory practices for handling sensitive biological materials.

Main Results:

Exogenous contamination is identified as the main weakness of PCR.
The tendency for false-positive results is directly linked to contamination.

Conclusions:

Contamination avoidance is the most critical factor for successful PCR.
Standard microbiological handling precautions are applicable and essential for PCR procedures.