Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biosensor-based methods in clinical diagnosis.

Methods in molecular medicine·2011
Same author

Characterization of a human pancreatic secretory trypsin inhibitor mutant binding to Legionella pneumophila as determined by a quartz crystal microbalance.

Journal of immunological methods·2000
Same author

Determination of phage antibody affinities to antigen by a microbalance sensor system.

BioTechniques·1999
Same author

Quartz crystal microbalance (QCM) as a device for the screening of phage libraries.

Biosensors & bioelectronics·1999
Same author

Characterization of immobilization methods for African swine fever virus protein and antibodies with a piezoelectric immunosensor.

Biosensors & bioelectronics·1999
Same author

[Oral hairy leukoplakia in human immunodeficiency virus positive patients: clinical and etiopathogenic aspects].

Acta otorrinolaringologica espanola·1992

Related Experiment Video

Updated: Jun 3, 2026

Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics
08:21

Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics

Published on: January 22, 2020

The Quartz Crystal Microbalance (QCM) as an Immunosensor.

F Aberl1, C Kößlinger, H Wolf

  • 1Securetec, Munich, Germany.

Methods in Molecular Medicine
|March 11, 2011
PubMed
Summary

This study presents a new method for detecting antiviral antibodies using a quartz crystal immunosensor. This technique offers continuous monitoring of molecular interactions, demonstrated through human immunodeficiency virus (HIV) antibody screening.

More Related Videos

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
07:11

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Published on: November 1, 2018

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Related Experiment Videos

Last Updated: Jun 3, 2026

Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics
08:21

Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics

Published on: January 22, 2020

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
07:11

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Published on: November 1, 2018

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Area of Science:

  • Biomedical Engineering
  • Immunotechnology
  • Biosensing

Background:

  • Immunoassays are crucial in clinical diagnostics for detecting specific antibodies.
  • Quartz crystal microbalances (QCMs) are acoustic immunosensors that detect molecular binding events.
  • Continuous monitoring of molecular interactions in liquid samples is essential for sensitive detection.

Purpose of the Study:

  • To demonstrate a preliminary experimental approach for detecting antiviral antibodies.
  • To present human immunodeficiency virus (HIV) specific antibody screening as a model application.
  • To explore the utility of quartz crystal immunosensors in clinical diagnostics.

Main Methods:

  • Utilizing a quartz crystal immunosensor based on acoustic shear wave propagation.
  • Coating the sensor surface with antigen to capture specific antibody molecules.
  • Monitoring changes in acoustic wave properties (phase and velocity) upon antibody binding.

Main Results:

  • The quartz crystal immunosensor successfully detected antiviral antibodies.
  • Continuous monitoring of molecular interactions was achieved.
  • The method was validated using human immunodeficiency virus (HIV) antibody screening.

Conclusions:

  • Quartz crystal immunosensors provide a viable method for detecting antiviral antibodies.
  • This biosensing approach offers a sensitive and continuous monitoring capability for immunoassays.
  • The presented technique holds potential for application in clinical diagnostics and disease screening.