Related Experiment Video
Updated: Jun 3, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
HPLC analysis of kaempherol and quercetin derivatives isolated by different extraction techniques from plant matrix
Krystyna Skalicka-Woźniak1, Janusz Szypowski, Kazimierz Głowniak
1Medical University of Lublin, Department of Pharmacognosy with Medicinal Plant Unit, 1 Chodzki St, 20-093 Lublin, Poland. kskalicka@pharmacognosy.org
Abstract:
Soxhlet extraction, ultrasound extraction, and accelerated solvent extraction (ASE), followed by RP-HPLC with a photodiode array detector was used for the determination of flavonoids in fruits of Peucedanum alsaticum. Three compounds were identified: a kaempherol derivative (astragalin), and two quercetin derivatives (quercitrin and hiperoside). The highest extraction yields of the selected compounds were obtained by use of exhaustive ASE.