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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...

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Controllable Ion Channel Expression through Inducible Transient Transfection
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A simple high-yielding process for transient gene expression in CHO cells.

Yashas Rajendra1, Divor Kiseljak, Lucia Baldi

  • 1Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

Journal of Biotechnology
|March 12, 2011
PubMed
Summary
This summary is machine-generated.

This study presents a simplified transient gene expression (TGE) method for Chinese hamster ovary (CHO) cells using reduced polyethylenimine (PEI) and DNA. This optimized bioprocess enhances cell viability and recombinant protein yields for efficient protein production.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Molecular Biology

Background:

  • Transient gene expression (TGE) is crucial for rapid recombinant protein production.
  • Chinese hamster ovary (CHO) cells are widely used in biopharmaceutical manufacturing.
  • Optimizing transfection protocols is key to improving cell viability and protein yield.

Purpose of the Study:

  • To develop a simplified and more efficient TGE method for suspension-adapted CHO cells.
  • To reduce reagent usage (PEI and plasmid DNA) while maintaining or improving protein yields.
  • To establish a scalable TGE process for recombinant protein production.

Main Methods:

  • Utilized polyethylenimine (PEI) for DNA delivery in suspension-adapted CHO cells.
  • Conducted transfection and production phases at a cell density of 4 × 10⁶ cells/mL and 31 °C.
  • Reduced PEI and plasmid DNA amounts by up to 50% per cell compared to previous methods.

Main Results:

  • Achieved higher cell viability post-transfection.
  • Obtained reproducible recombinant antibody yields up to 300 mg/L (small scale) and 250 mg/L (large scale) in 14-day batch cultures.
  • Demonstrated improved volumetric recombinant protein yields.

Conclusions:

  • The simplified TGE method enhances CHO cell utility for rapid recombinant protein production.
  • Reduced reagent concentrations improve cell viability and protein yields.
  • The optimized process is suitable for both small and large-scale TGE applications.