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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Childhood acute erythroleukemia diagnosis by flow cytometry
Anjali Sharma1, Gurdeep Buxi, Ritika Walia
1Department of Pathology, Dr. Ram Manohar Lohia Hospital and PGIMER, New Delhi 110 001, India. anjali27sharma@rediffmail.com
Indian Journal of Pathology & Microbiology
|March 12, 2011
Summary
This case study details a rare pediatric acute erythroid leukemia diagnosed using immunophenotyping. Flow cytometry confirmed the myeloid and erythroid nature of the blasts, aiding precise subtyping.
Area of Science:
- Hematology
- Pediatric Oncology
- Immunophenotyping
Background:
- Acute erythroid leukemia is a rare malignancy in pediatric patients.
- Accurate diagnosis and subtyping are crucial for effective treatment strategies.
Observation:
- A 1.5-year-old child presented with symptoms suggestive of leukemia.
- Initial diagnosis was based on peripheral smear, bone marrow examination, and cytochemistry.
Findings:
- Immunophenotyping confirmed acute erythroid leukemia.
- Flow cytometry revealed a blast population (29%) with variable CD45 expression.
- Blasts showed myeloid markers (cytoplasmic myeloperoxidase, CD117, CD13, CD33) and a strong erythroid marker (CD71).
Implications:
- Flow cytometry is a comprehensive tool for subtyping various leukemias and myelodysplastic syndromes.
- It can also differentiate neoplastic conditions from non-neoplastic mimics like reactive erythroid hyperplasia.
