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Related Concept Videos

Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic aliphatic amines show...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Amine Fragmentation00:55

Mass Spectrometry: Amine Fragmentation

Amines can be identified using mass spectroscopy based on their characteristic fragmentation patterns. The molecular ions of amines undergo fragmentation via ⍺-cleavage. The ⍺-cleavage of the carbon-carbon bonds in amines generates an alkyl radical and resonance-stabilized nitrogen-containing cation.
In amines, the number of nitrogen atoms affects the mass of the molecular ion, which is described by the nitrogen rule of mass spectrometry. This rule states that a compound containing a single or...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

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Published on: April 26, 2016

Amino Acid analysis by precolumn derivatization.

E L Harris1

  • 1Celltech Ltd., Slough, Berkshire, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 15, 2011
PubMed
Summary
This summary is machine-generated.

Amino acid analysis is crucial for biological and medical research. It enables the precise measurement of amino acids in various samples, aiding in research and diagnostics.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biomedical Research

Background:

  • Amino acid analysis is fundamental to understanding biological systems.
  • Accurate quantitation of amino acids is vital for numerous research applications.

Purpose of the Study:

  • To highlight the significance of amino acid analysis in biological and medical research.
  • To emphasize its role in characterizing and quantifying amino acids in diverse matrices.

Main Methods:

  • Utilizes various analytical techniques for amino acid separation and detection.
  • Applies methods for both free amino acids and those within protein/peptide structures.

Main Results:

  • Demonstrates the broad applicability of amino acid analysis across different sample types.
  • Confirms its utility in both qualitative and quantitative research endeavors.

Conclusions:

  • Amino acid analysis is an indispensable tool in modern biological and medical research.
  • Its application spans from fundamental science to clinical diagnostics and food science.