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A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
07:53

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Published on: January 9, 2019

Enzymatic methods for cleaving proteins.

B J Smith1

  • 1Celltech Ltd., Slough, Berkshire, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 15, 2011
PubMed
Summary
This summary is machine-generated.

Protein chemists fragment proteins for peptide mapping and structural studies. Limited specific proteinases yield complex peptide mixtures, hindering research.

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Last Updated: Jun 3, 2026

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Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

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Area of Science:

  • Biochemistry
  • Proteomics

Background:

  • Protein fragmentation is crucial for peptide mapping and primary structure determination.
  • Understanding protein structure-function relationships often requires specific peptide fragments.

Purpose of the Study:

  • To highlight the importance of protein fragmentation in biochemical research.
  • To discuss the challenges associated with generating pure peptide fragments using available proteinases.

Main Methods:

  • Utilizing endo-proteinases for controlled protein cleavage.
  • Analyzing the resulting peptide mixtures for purity and yield.

Main Results:

  • Limited availability of specific and effective proteinases restricts the generation of pure peptide fragments.
  • Incomplete or non-specific enzymatic cleavage results in complex polypeptide mixtures.

Conclusions:

  • The development of more specific proteinases is essential for advancing peptide mapping and structural biology.
  • Efficient protein fragmentation methods are critical for obtaining high-quality peptide samples for functional studies.