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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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The design, preparation, and use of immunopurification reagents

A Kenney1, L Goulding, C Hill

  • 1Downstream Processing Department, Celltech Ltd., Slough, Berkshire, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 15, 2011
PubMed

Immunoaffinity chromatography using immobilized monoclonal antibodies has become one of the most powerful macromolecular separation methods available. In many cases, simple one-step immunoaffinity separations will produce a several thousand-fold purification with yields of better than 80%. Monoclonal antibodies are now available with specificities for many different antigens, and the technology for producing new monoclonal antibodies has become widespread througout the world since its invention (1; see vol. 1 of this series). It has been estimated that a new monoclonal antibody is produced every 40 min worldwide.

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