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Updated: Jun 3, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries

Evangelia G Chronopoulou1, Nikolaos E Labrou1

  • 1Agricultural University of Athens, Athens, Greece.

Current Protocols in Protein Science
|March 15, 2011
PubMed
Summary

Site-saturation mutagenesis (SSM) enables rapid protein engineering by substituting amino acids at specific sites. This powerful PCR-based method efficiently generates diverse protein variants for structure-function studies.

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