Methyl-coenzyme M reductase from Methanothermobacter marburgensis
Related Experiment Video
Updated: Jun 3, 2026

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
Published on: January 31, 2025
Evert C Duin1, Divya Prakash, Charlene Brungess
1Department of Chemistry and Biochemistry, Auburn University, Alabama, USA.
Abstract:
Methyl-coenzyme M reductase catalyzes the reversible synthesis of methane from methyl-coenzyme M in methanogenic and ANME-1 and ANME-2 Archaea. The purification procedure for methyl-coenzyme M reductase from Methanothermobacter marburgensis is described. The procedure is an accumulation of almost 30 years of research on MCR starting with the first purification described by Ellefson and Wolfe (Ellefson, W.L., and Wolfe, R.S. (1981). Component C of the methylreductase system of Methanobacterium. J. Biol. Chem.256, 4259-4262). To provide a context for this procedure, some background information is provided, including a description of whole cell experiments that provided much of our knowledge of the behavior and properties of methyl-coenzyme M reductase.
More Related Videos
17:12Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
Published on: December 20, 2010
04:32Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
Published on: October 14, 2021
Related Concept Videos
Microbial Interactions: Mutualism
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Microbes and Methanogenesis
Electron Transport Chain: Complex III and IV
Chemotaxis in E. coli
Phase II Reactions: Methylation Reactions
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...