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Updated: Jun 3, 2026

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
Published on: October 14, 2025
[CRISPR-Cas system and coevolution of bacteria and phages]
1College of Life Science, Liaoning University, Shenyang 110036, China. tieminli@lnu.edu.cn
Abstract:
In response to the challenge from phages, bacteria evolve a number of defense systems against phage invasion. Meanwhile, the phages evolve multiple counter-defense mechanism as well under the selection pressure from bacteria. The evolution relationship between bacteria and phages, as well as the functional mechanism, still remains to be uncovered. A novel immune system, CRISPR-Cas system, has been found in bacteria and archaea recently. With deeply research of the function and mechanism of CRISPR-Cas system, the coevolution relationship between bacteria and phages is becoming clear. In this review, the mechanism of CRISPR-Cas system-mediated immunity in prokaryotes is introduced. In particular, the progress on the role of CRISPR in the coevolution of bacteria and phage is reviewed.
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