Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revealing the Innate Subnanometer Porous Structure of Carbon Nanomembranes with Molecular Dynamics Simulations and Highly-Charged Ion Spectroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same author

Advances in carbon nanomembranes for separation: from free-standing films to composite structures.

Nanoscale·2025
Same author

Publisher Correction: Elastic films of single-crystal two-dimensional covalent organic frameworks.

Nature·2024
Same author

Elastic films of single-crystal two-dimensional covalent organic frameworks.

Nature·2024
Same author

Defect-Healed Carbon Nanomembranes for Enhanced Salt Separation: Scalable Synthesis and Performance.

ACS applied materials & interfaces·2024
Same author

Anti-Arrhenius passage of gaseous molecules through nanoporous two-dimensional membranes.

Physical chemistry chemical physics : PCCP·2024
Same journal

The influence of water on the dynamics of alternating polymers P(C<sub>8</sub>EG<sub>4</sub>) and P(C<sub>4</sub>EG<sub>4</sub>) by broadband dielectric spectroscopy.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

How surface curvature shapes water nanodroplets in air.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

Topological boundaries in non-Hermitian p-wave Kitaev chains with Rashba spin-orbit coupling.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

Unravelling the local structure and magnetic dynamics of Cu-doped MnV₂O₄.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

Interplay of Anisotropy, Dzyaloshinskii Moriya Interaction and Symmetry breaking Fields in a 2D XY Ferromagnet.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

Single-molecule electron transport near a charge-trapping orbital-level alignment.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

Low energy electron point source microscopy: beyond imaging.

André Beyer1, Armin Gölzhäuser

  • 1Physics of Supramolecular Systems and Surfaces, University of Bielefeld, Bielefeld, Germany.

Journal of Physics. Condensed Matter : an Institute of Physics Journal
|March 16, 2011
PubMed
Summary
This summary is machine-generated.

Low energy electron point source (LEEPS) microscopy achieves 2 nm resolution, with potential for atomic-level imaging. Challenges include electric fields and charging, but LEEPS can also image electrical resistance in nanowires.

More Related Videos

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

Related Experiment Videos

Last Updated: Jun 3, 2026

A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

Area of Science:

  • Materials Science
  • Nanotechnology
  • Microscopy

Background:

  • Low energy electron point source (LEEPS) microscopy offers high magnification and simple setup for imaging.
  • Current resolution is approximately 2 nm, with theoretical potential for atomic-level detail.

Purpose of the Study:

  • To review achievements and challenges in advancing LEEPS microscopy resolution.
  • To explore applications of LEEPS microscopy beyond structural imaging.

Main Methods:

  • Numerical reconstruction of in-line holograms recorded by LEEPS microscopy.
  • Analysis of LEEPS micrographs to determine electrical resistance.

Main Results:

  • LEEPS microscopy can resolve structural features down to 2 nm.
  • Electric fields, charging, and radiation effects currently limit higher resolution.
  • LEEPS microscopy can be utilized for conductivity imaging of individual nanowires.

Conclusions:

  • Advancing LEEPS microscopy towards atomic resolution faces significant obstacles.
  • LEEPS microscopy offers valuable applications in conductivity imaging, extending its utility beyond structural analysis.