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Updated: Jun 3, 2026

Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)
Published on: September 5, 2025
[Comparison research on two-stage sequencing batch MBR and one-stage MBR]
Xin-Yan Yuan1, Heng-Gen Shen, Lei Sun
1College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China. pjxyxy@163.com
Two-stage sequencing batch MBR (TSBMBR) offers superior nitrogen and phosphorus removal and reduced membrane fouling compared to traditional one-stage aerobic MBR systems. This advanced MBR technology improves effluent quality for environmental use.
Area of Science:
- Environmental Engineering
- Water Treatment Technologies
- Membrane Bioreactors
Background:
- Membrane bioreactors (MBR) face challenges including low nutrient removal efficiency and severe membrane fouling.
- Traditional one-stage aerobic MBR systems exhibit limitations in nitrogen and phosphorus removal.
Purpose of the Study:
- To compare the performance of a two-stage sequencing batch MBR (TSBMBR) with a one-stage aerobic MBR.
- To evaluate nutrient removal efficiency and membrane fouling characteristics of both MBR configurations.
Main Methods:
- Comparative analysis of TSBMBR and one-stage aerobic MBR under steady operation.
- Monitoring of effluent nutrient concentrations (NH4(+)-N, TN, TP).
- Assessment of membrane fouling indicators, including soluble microbial products (SMP) and trans-membrane flux deduction rate.
Main Results:
- TSBMBR demonstrated significantly higher nitrogen and phosphorus removal efficiencies compared to one-stage aerobic MBR.
- Effluent concentrations reached levels suitable for domestic scenic environment use (Avg. NH4(+)-N: 2.83 mg/L, TN: 12.20 mg/L, TP: 0.42 mg/L).
- TSBMBR exhibited reduced membrane fouling, with lower SMP, slower trans-membrane flux deduction rates, and improved resistance to sedimentation and gel layer formation.
Conclusions:
- TSBMBR effectively addresses the limitations of traditional MBRs in nutrient removal.
- The TSBMBR system offers enhanced membrane performance with higher trans-membrane flux and lower fouling rates.
- TSBMBR presents a promising solution for efficient wastewater treatment with improved effluent quality and operational stability.
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