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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
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Systematic antibody and antigen-based proteomic profiling with microarrays.

Burcu Ayoglu1, Anna Häggmark, Maja Neiman

  • 1Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden.

Expert Review of Molecular Diagnostics
|March 17, 2011
PubMed
Summary

Affinity-based proteomics utilizes microarrays and reagents to discover novel protein-disease links in body fluids. This approach streamlines biomarker discovery and verification for clinical applications.

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Area of Science:

  • Biotechnology
  • Proteomics
  • Biomarker Discovery

Background:

  • Affinity-based proteomics relies on antigen/reagent availability and multiplexed technologies.
  • Planar microarrays and multiparallel systems enable hypothesis-driven and hypothesis-generating target selection.

Purpose of the Study:

  • To summarize current methods in array- and affinity-based proteomic profiling.
  • To provide a perspective on future developments for biomarker discovery in body fluids.

Main Methods:

  • Utilizing planar microarrays and multiparallel systems.
  • Employing affinity tools for target selection and verification.
  • Focusing on proteomic profiling of body fluids.

Main Results:

  • Discovery of new protein-disease associations is facilitated by combined technologies.
  • Verification of potential clinical relevance is crucial for discovered markers.
  • Affinity tools simplify the transition to clinically required assay formats.

Conclusions:

  • Array- and affinity-based proteomics offer a streamlined path for biomarker discovery.
  • Current and upcoming developments hold significant potential for advancing bioscience.
  • This approach facilitates the introduction of new markers for clinical use.