Zymodeme conversion of isolates of Entamoeba histolytica

A non-cloned and a cloned isolate of non-pathogenic (zymodeme I) Entamoeba histolytica derived from homosexual males were incubated in Diamonds' TP-S-1 medium in the presence of Crithidia for 52 d. A single addition of Escherichia coli irradiated at 1000 Gy rendered the culture axenic. Analysis of zymodeme patterns established that both isolates had transformed to zymodeme II. The re-establishment of cultures in the presence of microflora from the original cultures resulted in a return to zymodeme I, whereas incubation with microflora from an amoebic isolate exhibiting a zymodeme II pattern maintained the pathogenic pattern.