Related Experiment Video
Updated: Jun 3, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
The RP-Mdm2-p53 pathway and tumorigenesis
Paula L Miliani de Marval1, Yanping Zhang
1Department of Radiation Oncology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
The p53 tumor suppressor pathway is crucial for cell homeostasis. This study reveals that ribosomal protein interactions with Mdm2 are vital for p53
Area of Science:
- Molecular Biology
- Cell Biology
- Cancer Biology
Background:
- The p53 tumor suppressor is a key regulator of cellular homeostasis, responding to various stressors.
- Ribosomal protein (RP)-mediated suppression of Mdm2 E3 ligase activity activates p53.
- Mdm2 mutations disrupting RP binding are found in human cancers, but their significance is unclear.
Purpose of the Study:
- To investigate the in vivo physiological significance of the ribosomal protein-Mdm2 interaction in p53 signaling.
- To determine if disrupting RP binding to Mdm2 affects the p53 response to ribosomal biogenesis perturbations.
Main Methods:
- Generation of Mdm2C305F mutant mice with disrupted RPL11 and RPL5 binding.
- Assessment of p53 response to DNA damage and ribosomal biogenesis inhibition in Mdm2C305F mice.
Main Results:
- Mdm2C305F mice are viable and maintain a normal p53 response to DNA damage.
- Mdm2C305F mice exhibit a diminished p53 response to ribosomal biogenesis perturbations.
- This provides the first in vivo evidence for an RP-Mdm2-p53 signaling pathway.
Conclusions:
- The interaction between ribosomal proteins and Mdm2 is essential for the p53 pathway's response to ribosomal stress.
- This pathway's dysregulation may be relevant to human cancer development.
More Related Videos
04:12Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate (DMBA-TPA)
Published on: December 19, 2019
04:56Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
Published on: December 30, 2025
Related Concept Videos
Abnormal Proliferation
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Negative Regulator Molecules
PI3K/mTOR/AKT Signaling Pathway