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Co-regulation of the DAF-16 target gene, cyp-35B1/dod-13, by HSF-1 in C. elegans dauer larvae and daf-2 insulin

Wendy B Iser1, Mark A Wilson, William H Wood

  • 1Invertebrate Molecular Genetics Unit, Laboratory of Neurosciences, Research Resources Branch, NIA Intramural Research Program, NIH Biomedical Research Center, Baltimore, Maryland, United States of America.

Plos One
|March 17, 2011
PubMed

Insights

Insulin/IGF-I-like signaling (IIS) regulates cell functions. This study identifies new non-autonomous IIS targets in C. elegans, revealing a role for hsf-1 in mediating these effects.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Insulin/IGF-I-like signaling (IIS) governs cell-autonomous functions like growth and metabolism.
  • Non-autonomous IIS functions, crucial for processes like C. elegans dauer morphogenesis, have poorly understood targets.

Purpose of the Study:

  • To identify non-autonomous targets of C. elegans IIS using genomic and genetic methods.
  • To investigate the role of specific genes, such as cyp-35B1, in IIS pathways.

Main Methods:

  • Transcriptional microarrays to identify IIS-regulated genes in specific tissues (intestine, neurons).
  • Tissue-restricted IIS manipulation to analyze gene expression changes.
  • Genetic analysis of gene promoters, including DAF-16 and HSF-1 binding sites.

Main Results:

  • IIS differentially regulates stress response genes, including hsp-16, in a tissue-specific manner.
  • cyp-35B1/dod-13, a cytochrome P450 gene, is identified as a non-autonomous IIS target.
  • DAF-16 and HSF-1 are direct regulators of the cyp-35B1 promoter.

Conclusions:

  • hsf-1 is proposed to be involved in mediating the non-autonomous functions of age-1 in C. elegans.
  • Understanding these IIS targets provides insights into developmental and stress response pathways.

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