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Related Experiment Video

Updated: Jun 3, 2026

Electrophoretic Separation of Proteins

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Rotating Field Gel Electrophoresis (ROFE).

A Ziegler¹, A Volz

¹Institut für Experimentelle Onkologie und Transplantationsmedizin, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|March 17, 2011

Summary

Rotating field gel electrophoresis (ROFE) offers a novel approach for separating large nucleic acid molecules. This method utilizes a rotating electric field, providing an alternative to existing pulsed-field gel electrophoresis techniques.

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Electrophoretic Separation of Proteins

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Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Pulsed-field gel electrophoresis (PFGE) techniques like OFAGE, TAFE, and FIGE are used for separating large nucleic acid molecules.
These methods involve electronic switching between electric fields at specific angles.
A need exists for alternative methods offering comparable or improved separation capabilities.

Purpose of the Study:

To introduce and describe Rotating Field Gel Electrophoresis (ROFE) as a novel PFGE technique.
To present ROFE as a viable alternative to existing PFGE methods for large nucleic acid separation.
To explain the fundamental principles and apparatus of ROFE.

Main Methods:

ROFE employs a rotor carrying electrodes that rotate around a stationary gel.
The electric field is generated between main electrodes and stabilized by additional regulated electrodes.
The electric field direction is reoriented at predetermined intervals via rotor rotation.

Main Results:

ROFE achieves separation of very large nucleic acid molecules.
The separation principle in ROFE is analogous to that obtained with purely electronic switching PFGE methods.
ROFE provides an alternative mechanism for manipulating electric fields in gel electrophoresis.

Conclusions:

ROFE is a promising alternative to conventional PFGE techniques for separating large DNA molecules.
The rotating electric field mechanism in ROFE offers a distinct approach to molecular separation.
Further studies may explore the efficiency and resolution of ROFE compared to existing methods.