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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Jun 3, 2026

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
07:01

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Published on: April 11, 2015

Drop-on-demand single cell isolation and total RNA analysis.

Sangjun Moon1, Yun-Gon Kim, Lingsheng Dong

  • 1Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.

Plos One
|March 18, 2011
PubMed
Summary

This study introduces a microdroplet method for high-throughput single-cell isolation and patterning, ensuring high cell viability for genomic analysis. The automated platform streamlines cell manipulation for medical genomics applications.

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Last Updated: Jun 3, 2026

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
07:01

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

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Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Area of Science:

  • Biotechnology
  • Genomics
  • Cell Biology

Background:

  • Single-cell isolation is crucial for medical genomics but faces challenges in efficiency, viability, and control.
  • Existing methods for cell manipulation often involve multiple handling steps, impacting throughput and cell survival.

Purpose of the Study:

  • To develop and analyze a microdroplet-based method for efficient, high-viability single-cell isolation and patterning from heterogeneous cell suspensions.
  • To enable subsequent genomic analysis of isolated single cells and identify key biomarkers.

Main Methods:

  • A microdroplet generation system was employed for encapsulating single cells from mixed suspensions.
  • The cell encapsulation process underwent experimental and theoretical analysis.
  • The viability of isolated cells was assessed, and genomic information was obtained.

Main Results:

  • The microdroplet method achieved high single-cell isolation and patterning from a 10% target cell mixture.
  • Cell viability was preserved at a high rate of 97.0±0.8%.
  • Genomic analysis identified 11 stem cell markers among 1000 genes, outperforming non-patterned controls.

Conclusions:

  • The developed automated microdroplet platform offers an efficient and high-throughput solution for single-cell isolation and genomic analysis.
  • This method enhances cell viability and reduces handling steps compared to conventional techniques.
  • The platform facilitates biomarker discovery and advances applications in medical genomics.