Prostate cancer metabolite quantification relative to water in 1H-MRSI in vivo at 3 Tesla

(1)H magnetic resonance spectroscopic imaging was performed on 16 men with suspected prostate cancer using an 8-channel external receive coil at 3 T. Choline and citrate (Cit) signals were measured in prostate lesions and normal-appearing peripheral zone as identified on T(2)-weighted images. Metabolites were quantified relative to unsuppressed water from a separately acquired magnetic resonance spectroscopic imaging dataset using LCModel. Validation experiments were also performed in a phantom containing physiological concentrations of choline, Cit, and creatine. In vitro, fair agreement between measured and true concentrations was observed, with the greatest discrepancy being a 35% underestimation of Cit. In vivo, one dataset was rejected for failure to meet the quality criterion of linewidth <15 Hz, and in 6 of 15 subjects, insufficient normal-appearing peripheral zone tissue was identified for study. Lesions were found to have higher choline and choline/Cit, and lower Cit, than normal-appearing peripheral zone. The smaller skew of data obtained using water normalization in comparison with metabolite ratios suggests potential usefulness in longitudinal tumor monitoring and in studies of treatment effects.