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An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
Published on: October 11, 2022
Molecular detection and identification of Rickettsia species in Ixodes pacificus in California
Jimmy Ninh Phan1, Casey Roy Lu, William Garrett Bender
1Department of Biological Sciences, Humboldt State University, Arcata, California, USA.
Abstract:
We amplified 16S rRNA, gltA, and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus. While phylotype G021 has high homology to Ixodes scapularis endosymbiotic Rickettsia, phylotype G022 is a deeply branched novel spotted fever group Rickettsia.
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