Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Photovoltaics for space applications.

Communications engineering·2026

Same author

The effectiveness of a foot-care education program based on multi-theory model in diabetic patients with risk of foot ulceration: A randomized controlled trial protocol.

PloS one·2026

Same author

Red Jujube Juice Fermented with <i>Pediococcus pentosaceus</i> and Its Antioxidant Activity in C2C12 Cells.

Foods (Basel, Switzerland)·2026

Same author

AI-enhanced Wrist-Hand US Image Acquisition: Development and Initial Clinical Evaluation.

Radiology·2026

Same author

KIF11 drives ovarian cancer progression and cisplatin resistance via the Wnt/β-catenin signaling pathway.

Biochemical and biophysical research communications·2026

Same author

OrgLine: A versatile pipeline for organoid morphometry using detector-guided prompts.

Cell reports methods·2026

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

Published on: January 28, 2018

A carbon dots-based fluorescence turn-on method for DNA determination.

WenJun Bai¹, HuZhi Zheng, YiJuan Long

¹Key Laboratory on Luminescence and Real-Time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing, P. R. China.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|March 19, 2011

Summary

This study introduces a novel DNA detection system using carbon dots (CDots). These CDots

More Related Videos

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Published on: November 5, 2010

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: Jun 3, 2026

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

Published on: January 28, 2018

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Published on: November 5, 2010

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

Nanomaterials Science
Biochemistry
Analytical Chemistry

Background:

Carbon dots (CDots) exhibit fluorescence properties sensitive to their environment.
Methylene blue (MB) is known to quench the fluorescence of various nanomaterials.
Deoxyribonucleic acid (DNA) interactions can alter the properties of adsorbed molecules.

Purpose of the Study:

To develop a novel, sensitive method for deoxyribonucleic acid (DNA) detection.
To investigate the reversible fluorescence quenching of carbon dots (CDots) by methylene blue (MB).
To explore the mechanism of fluorescence restoration upon DNA addition.

Main Methods:

Utilizing carbon dots (CDots) as a fluorescent probe.
Employing methylene blue (MB) as a fluorescence quencher.
Investigating the interaction between CDots, MB, and deoxyribonucleic acid (DNA) via fluorescence spectroscopy.
Developing a quantitative detection assay for ct-DNA.

Main Results:

Methylene blue (MB) efficiently quenched the fluorescence of carbon dots (CDots).
Addition of ct-DNA caused a reversible restoration of CDots fluorescence.
A sensitive DNA detection system was established with a detection limit of 1.0 × 10⁻⁶ mol L⁻¹.
A linear detection range for ct-DNA was observed from 3.0 × 10⁻⁶ to 8.0 × 10⁻⁵ mol L⁻¹.

Conclusions:

Carbon dots (CDots) can be effectively used for sensitive deoxyribonucleic acid (DNA) detection.
The fluorescence quenching-restoration mechanism involving MB and DNA provides a basis for a new analytical platform.
This represents the first reported application of CDots for DNA detection.