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Primer extension analysis of mRNA isolated from transfected cell lines

M W Leonard1, R K Patient

  • 1King's College, University of London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2011
PubMed

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...